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Closed CLOSING DATE: 17/03/2019

Charles River Laboratories

SLSIP 2019 - Industrial Placement in Reg. ADME & Discovery Sciences Intern

0 Application(s) to date

Undergraduate Degree

Closing Date:
17/03/2019
Skill:
Science and environment
Region:
Edinburgh and Lothians
Salary
£9 ph
Length:
12 weeks
Ref code
BZREVGRVTD

Placement summary

The Department of Metabolism and Pharmacokinetics provides support to the pharmaceutical, veterinary pharmaceutical and chemical/agrochemical industries by evaluating the metabolism and disposition of new chemical and biological entities in vivo.  A key test method to help us achieve this is Quantitative Whole Body Autoradiography (QWBA).  When a radioactive-labelled drug is administered in vivo, this technique allows us to generate images of a whole biological system to assess exactly which tissues the drug is distributed to. This gives us valuable information as to whether the drug is going to desirable or undesirable areas of the body, and how long it stays there.

Human blood is currently used as a vehicle for radioactive standard controls as part of the QWBA technique. Blood provides an excellent comparison as a biological matrix to the test systems under analysis, however its supply relies on healthy volunteers and a phlebotomist, meaning that it is not instantly replenishable by the testing laboratory. The blood also provides a challenge during preparation due to blood clots and is time-consuming to prepare.

The aim of this project is to perform a comparative study to investigate the potential of a common emulsifier/thickener called carboxymethylcellulose (CMC) to replace human blood samples as a vehicle for radioactive control standards in QWBA tests.

Job description

Project Description

The placement holder will be responsible for accurate preparation of standard solutions of CMC and human blood. Following training, the post-holder will manipulate known amounts of radioactive isotopes, adding them to the standard solutions and preparing serial dilutions.

The successful candidate will create standard blocks which will be processed using a sophisticated cryomacrotome in order to generate micrometre thick sections for analysis.

Following section preparation, the post-holder will be responsible for the exposure of sections using specialised film. They will then perform image capture ahead of analysis of sections using the AIDA image analysis software.

The placement holder will be fully responsible for accurate data recording and documentation of all processes in accordance with the principles of Good Laboratory Practice (GLP).

In conjunction with the Lead Scientist, the placement will be responsible for analysing all data, making scientific judgements and recommending the next steps in order to achieve the aims of the project.

Placement Objectives

This project aims to validate the use of CMC to replace human blood as the vehicle for the standard controls. The project will assess whether the CMC has any impact on the ability of the QWBA technique to detect known amounts of radioactivity mixed into the vehicle. Success would be CMC performing comparatively to human blood under test conditions, which would allow QWBA to run more efficiently.

Role Description

The placement holder will be responsible for accurate preparation of standard solutions of CMC and human blood. Following training, the post-holder will manipulate known amounts of radioactive isotopes, adding them to the standard solutions and preparing serial dilutions.
 
The successful candidate will create standard blocks which will be processed using a sophisticated cryomacrotome in order to generate micrometre thick sections for analysis.
 
Following section preparation, the post-holder will be responsible for the exposure of sections using specialised film. They will then perform image capture ahead of analysis of sections using the AIDA image analysis software.
 
The placement holder will be fully responsible for accurate data recording and documentation of all processes in accordance with the principles of Good Laboratory Practice (GLP).
 
In conjunction with the Lead Scientist, the placement will be responsible for analysing all data, making scientific judgements and recommending the next steps in order to achieve the aims of the project.
 

Qualifications

This programme is for 1st, 2nd or 3rd year undergraduate students studying a Life Science degree in Scotland. Please click here to see if your course is eligible. You can only apply for a maximum of 3 placements, so please research the roles thoroughly before submitting your CV.

Skills / Experience

Essential Skills

  • Knowledge and understanding of biology or chemistry 
  • Basic laboratory skills
  • Preparing chemical solutions
  • Basic mathematical ability

Desirable Skills

  • Attention to detail
  • Strong communication
  • Knowledge of laboratory health and safety

Benefits

Placement benefits

Charles River will provide training in the necessary laboratory techniques to ensure safety and quality while in the laboratory.

The project will teach key industry skills in Good Laboratory Practice (GLP), providing an insight into compliance with regulatory standards. The placement-holder will gain experience in the performance of a study from set up to delivery of results, learning the documentation skills necessary for industry.

Charles River Edinburgh has a canteen offering hot and cold food and an onsite fitness suite as well as free staff parking on-site and transport links from Edinburgh.

Additional information

Tranent

01/04/2019

01/06/2019

Company information

Charles River is committed to helping our partners expedite their preclinical drug development with exceptional safety assessment services, state-of-the-art facilities and expert regulatory guidance. Each year approximately 120 investigational new drug (IND) programs are conducted in our Safety Assessment facilities.

Our Edinburgh research campus has circa 1,100 staff situated in the countryside 15 miles east of Edinburgh. We can offer you the chance to build on your skills and knowledge with career enhancing experience and work with world-class experts.

Life Sciences

Company website: www.criver.com